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Quantitative estimation of dehydroascorbic acid and ascorbic acid by HPLC - Application to human milk plasma and leukocytes
Summary
Type:
HPLC
Application #:
109630
Catalog#:
720018.40 - MN HPLC Column, Nucleosil C18, 100A, 7um, 4x250mm
Detection:
UV, 254nm
Conditions:
eluent: 2mM tetrabutylammonium hydroxide pH 2.92
flow rate 1.5ml/min
Authors:
Nyyssönen K
Source:
J Liquid Chromatogr 11 (1988) 1717 - 1728
Details
Legend: Fig. 1. Chromatograms of 20 µl injections of a) ascorbic acid standard, b) ascorbic acid in filtered protein-free milk, c) homocysteine-treated dehydroascorbic acid standard and d) a homocysteine-trea
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